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pdgfaa  (R&D Systems)


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    R&D Systems pdgfaa
    Pdgfaa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pdgfaa/product/R&D Systems
    Average 95 stars, based on 134 article reviews
    pdgfaa - by Bioz Stars, 2026-04
    95/100 stars

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    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated with PDGF-AA or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.

    Journal: bioRxiv

    Article Title: INPP5E interactome reveals novel connections to growth factor signaling

    doi: 10.64898/2026.02.13.705725

    Figure Lengend Snippet: (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated with PDGF-AA or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.

    Article Snippet: Other reagents included GFP-Trap_MA beads (Proteintech, gtma), mouse anti-Flag M2 magnetic beads (Sigma, M8823), sodium orthovanadate (Alfa Aesar, J60191) and the following recombinant human growth factors: PDGF-AA (Proteintech, HZ-1215), TGF-β1 (R&D Systems, 240-B), Insulin (Gibco, 12585014), IGF-I (Proteintech, HZ-1322) and EGF (Proteintech, HZ-1326).

    Techniques: Western Blot, Control, Cell Culture, Marker